detection of transplantation antigens by the Macrophage Migration Inhibition technique.

Detection of transplantation antigens by the Macrophage Migration Inhibition technique by Bruce Lance Gibbins Download PDF EPUB FB2

In animal studies, the technique has been successfully applied for the detection of transplantation antigens and cell-associated tumor antigens, as well as soluble tumor antigens.

It has been shown that this reaction is mediated by a soluble factor, produced by sensitized lymphocytes upon stimulation by specific antigen, that is, migration. The capillary tube method for studying the inhibition of macrophage migration in vitro has been widely shown to be a useful correlate of delayed-type hypersensitivity in experimental animals.

Recent reports indicate its potential usefulness in man. In animal studies, the technique has been successfully applied for the detection of transplantation antigens and cell-associated tumor antigens Cited by: Kronman BS, Wepsic HT, Churchill WH, Jr, Zbar B, Borsos T, Rapp HJ. Tumor-specific antigens detected by inhibition of macrophage migration.

Science. Jul 18; ()– Malmgren RA, Holmes EC, Morton DL, Yee CL, Marrone J, Myers MW. In vitro detection of guinea pig alloantigens by the macrophage-inhibition technique. by:   CELLULAR IMMUNOL () Antigen-Mediated Macrophage Adherence Inhibition V. HOLÄN, M. HASEK, J. BUBENiK AND JITKA CHUTNA Institute of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, 00 Prague, Czechoslovakia Received Decem Antigen-mediated macrophage adherence inhibition (MAI) was studied in inbred rats immunized with various transplantation Cited by: Macrophages in Ischemia Reperfusion Injury.

Ischemia reperfusion injury (IRI) is a multifactorial process, involving both innate and adaptive immunity, which impacts early and late graft dysfunction [].Cells of the innate immune system, particularly macrophages, are key potentiators of IRI, participating in both the early stages of injury and in late stage repair [21–25].

Studies of the macrophage-inhibition test. Use of the macrophage migration-inhibition technique in detection of active graft immunity. Ferraresi RW, Goihman-Yahr M, Raffel S. Transplantation, 10(3), 01 Sep Cited by: 2 articles | PMID: Tumor-specific antigens detected by inhibition of macrophage migration.

Science. Jul 18; ()– [Google Scholar] Malmgren RA, Holmes EC, Morton DL, Yee CL, Marrone J, Myers MW. In vitro detection of guinea pig alloantigens by the macrophage-inhibition technique. Transplantation. Oct; 8 (4)– [Google Scholar]. Purchase Clinical Tumor Immunology - 1st Edition.

Print Book & E-Book. ISBNThe Use of Cultured Lymphoid Cells as Target Cells for the Detection of Migration Inhibitory Factor An m Vitro Test for Delayed Hypersensitivity: Macrophage Spreading Inhibition (M.S.I.) The Technique for Observation of Migration and Aggregation of Human Blood Macrophages The in Vitro Macrophage Aggregation Assay for Delayed.

SPECIFIC antigen markedly inhibits the migration of macrophages from tissue explants of animals which show positive delayed hypersensitivity skin tests to that antigen1,2. The technique of. A leucocyte migration technique for in vitro detection of cellular (delayed type) hypersensitivity in man.

Dan. Med. Bull. [Medical Depts. P and A, Rigshospitalet, Univ. Copenhagen, Denmark] A capillary tube migration technique for investigation of specific antigen-induced migration inhibition of human peripheral blood cells is.

Transplantation of solid organs between genetically distinct individuals leads, in the absence of immunosuppression, to T cell-dependent transplant rejection.

Activation of graft-reactive T cells relies on the presentation of transplant-derived antigens (intact donor MHC molecules or processed peptides on host MHC molecules) by mature dendritic cells (DCs).

Allen, L. Davies, in Blood and Tissue Antigens, Discussion. Experimental work on human HL-A transplantation antigens is restricted by the special requirements imposed by this particular animal species. The mouse H–2 model has provided information that has not been so difficult to check with human material and the two systems prove to be so similar that genetic homology seems likely.

In vitro detection of transplantation immunity: the inhibition of migration of immune spleen cells and peripheral blood leukocytes by specific antigen. Falk RE, Collste L. In brucella hypersensitivity a specific, antigen-induced inhibition of the in vitro migration of peripheral human leucocytes has been shown, in the same experimental system, to be a parameter of.

transplantation antigen: an antigen on the surface of nucleated cells, particularly leucocytes and thrombocytes. See also: H-2 antigens. Synonym(s): transplantation antigen.

Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase is a protein that in humans is encoded by the MIF gene.

MIF is an important regulator of innate immunity. The MIF protein superfamily also includes a second member with functionally related properties, the D-dopachrome tautomerase (D-DT). Macrophage migration inhibition tests Mass cultivation of B.

melitensis Rev I for preparing soluble antigens was done by the method described by Van Drimmelen () for B. abortus SI9. Two soluble protein antigens were employed, one of which was a cold NaCl extract prepared by the.

Rouveix, B., Badenoch-Jones, P. and Turk, J. A sensitive technique for measuring macrophage aggregation: a comparison with macrophage migration inhibition for the detection of lymphokine activity.

Immunology, 36, PubMed Google Scholar. Macrophages also adapt their migration mode to the matrix architecture and the biophysical parameters of the collagenous matrix. For example, fibrillar type I collagen favors an amoeboid migration mode, while denser 3D collagen I matrices promote a mesenchymal migration mode of human monocyte-derived macrophages (47).

Abstract. The search for antigens on human neoplasms which evoke immune responses in the tumour-bearing host has been motivated by the unequivocal demonstration of such entities in many experimental tumours, using assays of both cellular and humoral immunity 1, seems implausible that human tumours should be exceptional in this regard, although one might anticipate that the variability of.Macrophage migration inhibition has been induced with cultures of normal guinea-pig peritoneal exudate cells and mitochondrial antigen of guinea-pig liver.

The effect, like the antigen induced phenomenon, is prevented by exposure to trypsin. Puromycin and cycloheximide in contrast did not prevent macrophage migration inhibition.Abstract. A specific macrophage migration inhibition assay, using patient lymphocytes incubated with a human colonic cancer extract, was studied in 92 patients with proven colorectal cancer and in other individuals (20 normal controls, 80 patients with various nonmalignant gastrointestinal diseases, and 34 patients with extracolonic malignancies).